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System Integration & Point of Care Devices

Human and animal health are currently in a transition, where evidence-based rapid therapeutic intervention and self-monitoring for lifestyle optimisation are on the rise. Diagnostic technology will be provided at the time and place where it is needed. The key driver for this development are point of care (POC) devices providing decentralized rapid testing.

Those systems depend on miniaturised and automated processes with superior analytical performance, enabling earlier and more accurate disease detection and prevention. AIT has profound expertise in the whole analysis chain ranging from sample preparation, assays, biosensors, microfluidics, process control and data analysis towards the overall POC instrument. This system know-how enables us to develop single analysis modules, provide content for external POC platforms and to integrate all components into fully functional devices providing simple, fast, and user-friendly operation. With such systems home-testing and self-monitoring can be performed, enabling lifestyle optimisation and early detection for the prevention of diseases. In addition, these systems will facilitate rapid diagnostic answers directly at the patient or animal and empower health professionals for better, targeted and personalised treatment.


  • Development of POC-compatible sample preparation methods with minimal handling steps based on enzymatic, thermal, chemical and mechanical lysis
  • POC-compatible assay design based on in-silico verification
  • POC-compatible assay development and process definition providing:
  • Rapid DNA, RNA, protein and metabolite analysis
  • Direct approaches for crude samples (e.g. directPCR)
  • Reagent storage and small reaction volumes
  • Simple operation protocols and minimal user intervention
  • Compact dimensions of analysis cartridges and devices
  • Assay transfer to POC platforms including optimisation
  • Development of quality controls for analyte extraction, reaction and readout processes
  • Integration of biochemical workflows into cartridges including sensors and actuators
  • Integration of external technologies with AIT‘s know-how
  • Validation of POC systems (in cooperation with clinical partners in human and veterinary medicine)


  • Acceleration of existing sample preparation protocols based on chemical, enzymatic or mechanical methods for a broad range of samples (e.g. blood, saliva, feces, urine)
  • In-silico assay design for DNA/RNA-based pathogen detection
  • Development and optimization of RNA, DNA, protein and metabolite assays towards POC-compatibility using qPCR/PCR, enzymatic and colorimetric assay technologies
  • Transfer of existing assays to POC platforms including adaptation and optimisation
  • Reagent storage and handling on POC systems
  • Process control and automation of extraction and measuring protocols
  • Design and development of extraction and assay controls (e.g. extraction control with specifically selected microorganisms)
  • Design and prototyping of microfluidic chips and cartridges in polymer substrates (e.g. micromilling, laser cutting)


  • Infectious disease diagnostics in the human and veterinary field
  • Rapid tests for mobile laboratories
  • Respiratory infection diagnostics for general practitioners
  • Oral disease monitoring for dentists
  • Monitoring of nutrition and stress parameters for individuals

Selected Projects

DECISION https://cordis.europa.eu/project/id/101005111   
MOBILISE https://cordis.europa.eu/project/id/101073982   
DIAGORAS https://www.diagoras.eu/   

Selected Publications

  • Paque PN, Herz C, Jenzer JS, Wiedermeier DB, Attin T, Bostanci N, Belibasakis GN, Bao K, Körner P, Fritz T, Prinz J, Schmidlin PR, Thurnheer T, Wegehaupt FJ, Mitsakakis K, Peham JR. Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay. (2020). Journal of Clinical Medicine, 1 (2020), 1:1-18, doi: 10.3390/jcm9092945 
  • Couperus A, Schroeder F, Hetteger P, Huber J, Wittek T, Peham J. Longitudinal Metabolic Biomarker Profile of Hyperketonemic Cows from Dry‐Off to Peak Lactation and Identification of Prognostic Classifiers. (2021). Animals, 11 (2021), 1353, 1-15, doi: 10.3390/ani11051353
  • Zirath H, Schnetz G, Glatz A, Spittler A, Redl H, Peham JR. Bedside Immune Monitoring: An Automated Immunoassay Platform for Quantification of Blood Biomarkers in Patient Serum within 20 Minutes. (2017) Analytical Chemistry, 89(9): 4817-4823, doi: 10.1021/acs.analchem.6b03624 
  • Wassermann KJ, Barth S, Keplinger F, Noehammer C, Peham JR. High-k Dielectric Passivation: Novel Considerations Enabling Cell Specific Lysis Induced by Electric Fields. (2016). Applied Materials and Interfaces, 8(33): 21228-21235, doi:10.1021/acsami.6b06927